排列三跨度走势图

返回排列三跨度走势图 联系我们
公司动态
当前位置:排列三跨度走势图 > 公司动态 > 实验室的PCR污染问题如何处理
实验室的PCR污染问题如何处理
点击次数:865 发布时间:2017-09-28

SHENGWUSHIYAN,DADUOSHUSHIYANJIANCEDOUSHIWEIGUANDE,JIYUXIBAO,FENZI,DANBAISHUIPING,ERZHEIXIEWEIGUANYANGBENDUIWAIJIEHUANJINGDEYINGXIANGMINGAN,BIRUWENDU、phZHI、MEIJIE、SUNSHANGDENG,ZAIWOMENJINLIHEHUYANGBENDESHENGCUNHUANJINGDETONGSHI,WURANYESHIXUYAOZHUYIDETOUDENGDASHI,YAOJIANJUEBIMIANHUANJINGZHONGDE“ZAZHI”DELUANRU,BAOKUOYANGBENWURAN,SHIJIYIQIJIAOCHAWURAN、CHANWUQIRONGJIAOWURANDENGDUISHIYANJIEGUODEYINGXIANG。“YICHABUYICHA”,WURANLAIDEQIAOWUSHENGXI,FANGBUSHENGFANG,JIAYANGXINGJIEGUORANGWUSHUSHENGWUXUEZIZHUAKUANGHEBENGKUI。

排列三跨度走势图ZAIZHONGDUODESHENGWUSHIYANSHI,FENZISHENGWUXUEJIANCEFANGFARUPCRYINQIGAOLINGMINDU、KUAISU、CAOZUOFANGBIANDENGYOUSHIYIJINGBEIGUANGFANYINGYONG,BUGUOZHENGSHIYOUYUTALINGMINDUJIGAO,JIWEILIANGDEWURANYUANDOUYOUKENENGDAOZHIJIANCEJIEGUODEJIAYANGXING,SUOYIDUIHUANJINGWURANDEKONGZHIYEJIWEIYANGE。MIANDUIZHONGDUODEWURANYUAN,BIRUYANGBENJIAOCHAWURAN、SHIJIYIQIJIAOCHAWURAN、KELONGZHILIWURAN、PCRKUOZENGCHANWUWURAN、HAIYOUJIRONGYIHUSHIDEQIRONGJIAOWURAN,QIRONGJIAOSHIYOUGUTIHUOYETIXIAOZHIDIANFENSANBINGXUANFUZAIQITIJIEZHIZHONGXINGCHENGDEJIAOTIFENSANTIXI,ZAIKONGQIYUYETIMIANMOCASHI,BIRUZAICAOZUOSHIBIJIAOJULIEDIYAODONGFANYINGGUAN,KAIGAISHI、XIYANGSHIJIWURANJINYANGQIANGDEFANFUXIYANGDOUKEXINGCHENGQIRONGJIAOERWURAN。JUJISUANYIGEQIRONGJIAOKELIKEHAN48000KAOBEI,YINERYOUQIZAOCHENGDEWURANSHIYIGEFEICHANGZHONGYAOERBEIHUSHIDEWURANYUAN。

SUIRANZHEIXIEWURANDUIPCRSHIYANJIEGUODEYINGXIANGYIJINGSHOUDAOLEGUANGFANZHONGSHI,DUOSHUSHIYANSHIDUIWURANKONGZHIDOUCAIQULEGEZHONGBANFA,BIRUDINGQIDASAOCHUQINGLI、HUAFENCAOZUOQUYU、SHIJIFENZHUANG、CAOZUOJINSHEN、ZHONGFUSHIYAN、DUOJIAODUYANZHENGJIEGUO。DANSHIWEILIANGDEHESUANPIANDUANJIUNENGBEIKUOZENG,SUOYIDUIWURANWUDEKONGZHIBUJINJINSHIZUODAOZHEIXIEJIUNENGGOUDUJUEHEBIMIANDE,GUANJIANSHIXUYAOCONGYUANTOUKONGZHI。MUQIANDUOSHUSHENGWUSHIYANSHI,FENZISHIYANMEITIANDOUZAIDAGUIMOJINXING,WURANYUANYIJINGWUCHUBUZAI,TEYIXINGDE,FEITEYIXINGDE,TONGYUANDE,FEITONGYUANDE,GUANGFANFENBUZAIYANGBENRONGQI、SHIYANSHITAIMIAN,SHIJIRONGYEZHONG、YIQIBIAOMIANNEIGUANFUZHEDENGDENG,NIYIWEIPUTONGDEQINGLIJIUNENGJIEJUEZHEIXIEWURANYUANMA?BIXUSHIBUXINGDE,SHIYANSHIDANAMENDOUWEILEQINGCHUZHEIXIEWURANYUANYANJIUCHUHAODUOZHONGFANGFA,ZHENGLIGENDAJIAGONGXIANG。

传统方式

原理

优势

不足

JIUJINGCASHI

JIANGHESUANCHENDIAN,CASHIHOUBAOZHENGWURANBIAOMIANWURANDEDAOYIDINGCHENGDUHUANJIE

JIANDAN、FANGBIAN、CHENGBENDI

BUCHEDI(HESUANPIANDUANBENSHENMEIYOUBIANHUA,RENGRANCUNZAIDAOZHIQIRONGJIAOWURANDEKENENGXING)、XIXIAOKONGJINGQICAIWUFAQINGCHU

XIUSHI

BIAOJIYICHUANWUZHI,ZUZHIJUHEMEIYUHESUANDEJIEHE,CONGERYIZHIDNAKUOZENG

XIAOGUOXIANGDUIHAO

FANYINGKENI、CHENGBENGAO、SHIJIKENENGJUYOUBUANQUANYINGXIANG

MEIJIE

MEIKEJIANGZHANGLIANDEHESUANFENZIJIANGJIECHENGXIAOPIANDUAN

SUDUKUAI,XIAOGUOXIANGDUIJIAOHAO

JIANGJIEDEHESUANZHONGRENGYOUDAPIANDUANCUNZAI,JUYOUWANZHENGDEYICHUANXINXI,KETONGGUOPCRKUOZENGCHUWANZHENGDEPIANDUAN、CHENGBENGAO

GAOYABIANXING

GAOYAKEJIANGHESUANJIANGJIECHENG20~30bpDEXIAOPIANDUAN

CHEDI,CHENGBENDI

排列三跨度走势图JINSHIYONGYUNAIGAOYACAILIAO,GENGWUFAYINGYONGSHIYANCAOZUOTAIHEDAXINGDESHIYANSHEBEI

ZIWAIZHAOSHE

排列三跨度走势图PCRCHANWUZHONGMIDINGJIANJIHUIXINGCHENGERJUTI,YANSHENZHONGZHI

FANGBIAN,FANWEIGUANG,CHENGBENDI

排列三跨度走势图JINDUI500bpYISHANGZHANGPIANDUANYOUXIAO,DUIDUANPIANDUANXIAOGUOBUDA,QIEBINGBUSHIDNALIANZHONGSUOYOUMIDINGJUNNENGXINGCHENGERJUTI,QIEUVZHAOSHEHAIKESHIERJUTIDUANLIE

纵然有这么多解决核酸污染源的办法,但是大多数实验室却由于使用不方便、操作复杂、成本高、危害大而没有持续采用,实验室累积的污染也就越来越多,有些实验者甚至在ddH2O中扩增出了条带,结果可重复性差、投稿文章被质疑,不得不重新实验,当然在实验之前,必须要彻底清除这些污染源,这么严重的污染如何的清除,是一个迫在眉睫的问题。目前国外学者经过不断研发拓展,有一款清除剂DNA-ExitusPlus已经问世,并得到了Nature Methods的推荐,此款清除剂纯绿色配方,能够将污染源的核酸片段彻底降解成核苷酸为单位的极小片段,此过程不可逆,避免了污染重复的可能性,使用方便,使用与所有台面,仪器擦拭,小部件浸泡等,无污染,无腐蚀性,对人体无危害。简直是广大核酸污染受害者的福音啊。